Cycloheximide Production by Streptomyces griseus : Control Mechanisms of Cycloheximide Biosynthesis

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Cycloheximide production by Streptomyces griseus: control mechanisms of cycloheximide biosynthesis.

Cycloheximide accumulation in a fermentation medium has been shown to be the product of the balance between synthesis and degradation of this antibiotic. Glucose has been shown to prevent cycloheximide degradation. Cycloheximide has been shown to interfere with its own synthesis probably due to feedback inhibition. Approaches for increasing cycloheximide titers in the light of these findings ar...

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Cycloheximide production by Streptomyces griseus: alleviation of end-product inhibition by dialysis-extraction fermentation.

The use of dialysis fermentation with the continuous extraction of the dialysate has resulted in a twofold increase in the cycloheximide titer due to relief from product inhibition. Continuous extraction of the dialysate has eliminated the necessity for large reservoir volumes of fermentation medium normally used in dialysis fermentation. The apparatus used in this procedure is described.

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Changes in the protein profile of Streptomyces griseus during a cycloheximide fermentation.

The production of the antifungal agent cycloheximide by Streptomyces griseus is typical of many antibiotic fermentations. In the initial stage of the fermentation, cell growth is extensive, while little antibiotic is produced. After roughly 24 hours, the biomass concentration remains relatively constant and accumulation of product in the fermentation broth begins. It has been shown that product...

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Oxidation of Meloxicam by Streptomyces griseus

The aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. Among the actinomycetes screened, Streptomyces griseus NCIM 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in S. griseus NCIM 2623...

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Effect of cycloheximide on protein biosynthesis in rat liver.

1. The liver ribosomes of rats given cycloheximide by intraperitoneal injection incorporate less amino acid into protein than ribosomes from control rat liver when they are incubated in vitro with excess of Sephadex-treated cell sap. The effect is rapid, marked and persistent. 2. Cell sap from liver of cycloheximide-treated animals is inhibitory but the inhibition can be relieved almost entirel...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1975

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.7.6.856